Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard.

نویسندگان

  • Sarka Bidmanova
  • Mark-Steven Steiner
  • Martin Stepan
  • Kamila Vymazalova
  • Michael A Gruber
  • Axel Duerkop
  • Jiri Damborsky
  • Zbynek Prokop
  • Otto S Wolfbeis
چکیده

Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote exists, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test strip for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the strips whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 μg/L, and the one for red-green-blue read-out is as low as 3 μg/L. The assays have good reproducibility ±6% and ±2% for interday assays and intraday assays, respectively. The strips can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Competitive immunochromatographic assay for the detection of thiodiglycol sulfoxide, a degradation product of sulfur mustard.

An immunochromatographic assay (ICA) based on the competitive antigen-coated format using colloidal gold as the label was developed for the detection of thiodiglycol sulfoxide (TDGO), an important metabolite and degradation compound of sulphur mustard (SM). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated wit...

متن کامل

Sulfur Mustard Exposure and Non-Ischemic Central Retinal Vein Occlusion

A 41-year-old man was referred with a complaint of visual loss in his left eye and his best corrected visual acuity was 20/80. Slit lamp examination showed arborizing conjunctival vessels and dry eye. Fundus examination and fluorescein angiography revealed a non-ischemic central retinal vein occlusion. Cardiovascular, rheumatologic, and hematologic work up showed no abnormal findings. An ascert...

متن کامل

Clinical Efficacy of Topical Avena sativa Versus Betamethasone in Chronic Pruritus due to Sulfur Mustard Exposure

Background: Avena sativa, a well-known herbal medicine; has been used in various skin diseases such as eczema, burn and pruritus. Objective: The objective of this study was to evaluate the effect of this herbal medicine for treatment of chronic pruritus in Sulfur Mustard (SM) exposed patients. Methods: Veterans who referred to Baghiat-Allah dermatologic clinic for itching problems were examin...

متن کامل

THE EFFECT OF SULFUR MUSTARD ON GLUCOSE PHOSPHORYLATING ENZYMES AND LIVER CELLS IN MALE RATS

A significant decrease of hepatic high-Km glucose- phosphorylating enzyme glucokinase (ATP- D- glucose 6- phosphotransferase, EC 2.7. 1.2, GK) and low-Km hexokinases (EC 2.7. 1.1. HK) activities were noticed in liver treated with 2, 2'- dichlorodiethyl sulfide (sulfur mustard, HD) when injectedinteraperitoneally to male rats in 114 LD50. This decrease occurred 1.5 to 5.5 hours after the in...

متن کامل

Effectiveness and Health Investigation of N-Acetyl Cysteine Tablets and Comparing it with Foreign Samples in Controlling Pulmonary Symptoms Caused by Sulfur Mustard in Chemical Veterans of Qazvin Province

Background and Aim: Sulfur mustard (Mustard gas) is the most famed chemical warfare agent that caused chronic lung damage. Oxidative stress is known as a major cause of mustard lung pathogenesis. This study aimed to introduce antioxidant tablets of Iranian N-acetyl-cysteine (NAC) as an effective treatment for chronic lung damage caused by mustard gas. Methods: This study was performed as a doub...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Analytical chemistry

دوره 88 11  شماره 

صفحات  -

تاریخ انتشار 2016